In the case of a form submission, if you have a template that includes specific subtasks that need to be assigned to particular individuals or teams, the automation of applying that template of subtasks and assignees, will overwrite the parent task assignee if filled out in the form.
Example: Approval subtasks assigned to particular teams, the request is submitted by someone not included or foreseen in the template. When the automation runs and applies the template, since the template must include "Assignees" it will overwrite and remove the requester assignee field selected.
It would be great to have a template that can distinguish the selections it applies for parent tasks vs. subtasks. This would create a completely customizable and dynamic workflow.